diff --git a/Lib/test/test_profiling/test_sampling_profiler.py b/Lib/test/test_profiling/test_sampling_profiler.py
index 59bc18b9bcf14d..ff4556a3d9400c 100644
--- a/Lib/test/test_profiling/test_sampling_profiler.py
+++ b/Lib/test/test_profiling/test_sampling_profiler.py
@@ -1740,8 +1740,8 @@ def main_loop():
         if iteration % 3 == 0:
             # Very CPU intensive
             result = cpu_intensive_work()
-        elif iteration % 5 == 0:
-            # Expensive recursive operation
+        elif iteration % 2 == 0:
+            # Expensive recursive operation (increased frequency for slower machines)
             result = slow_fibonacci(12)
         else:
             # Medium operation
diff --git a/Misc/NEWS.d/next/Tests/2025-10-27-14-24-18.gh-issue-140381.aA6MJa.rst b/Misc/NEWS.d/next/Tests/2025-10-27-14-24-18.gh-issue-140381.aA6MJa.rst
new file mode 100644
index 00000000000000..a6e0f2b4ccc016
--- /dev/null
+++ b/Misc/NEWS.d/next/Tests/2025-10-27-14-24-18.gh-issue-140381.aA6MJa.rst
@@ -0,0 +1 @@
+Misc/NEWS entry: Fix flaky test_profiling tests on i686 and s390x architectures by increasing slow_fibonacci call frequency from every 5th iteration to every 2nd iteration.
diff --git a/Misc/NEWS.d/next/Tests/2025-10-27-14-32-22.gh-issue-140381.N5o3pa.rst b/Misc/NEWS.d/next/Tests/2025-10-27-14-32-22.gh-issue-140381.N5o3pa.rst
new file mode 100644
index 00000000000000..568a2b65d7d204
--- /dev/null
+++ b/Misc/NEWS.d/next/Tests/2025-10-27-14-32-22.gh-issue-140381.N5o3pa.rst
@@ -0,0 +1 @@
+Fix flaky test_profiling tests on i686 and s390x architectures by increasing slow_fibonacci call frequency from every 5th iteration to every 2nd iteration.
diff --git a/Misc/NEWS.d/next/Tests/2025-10-27-15-53-47.gh-issue-140381.N5o3pa.rst b/Misc/NEWS.d/next/Tests/2025-10-27-15-53-47.gh-issue-140381.N5o3pa.rst
new file mode 100644
index 00000000000000..568a2b65d7d204
--- /dev/null
+++ b/Misc/NEWS.d/next/Tests/2025-10-27-15-53-47.gh-issue-140381.N5o3pa.rst
@@ -0,0 +1 @@
+Fix flaky test_profiling tests on i686 and s390x architectures by increasing slow_fibonacci call frequency from every 5th iteration to every 2nd iteration.